Development of Methods for the Multiple Synthesis and Analysis of Peptides and Peptide Libraries and for Combinatorial Organic Chemistry

The rapidly growing demand for synthetic peptides in research and development requires methods for the fast, simultaneous synthesis and analysis of hundreds of chemically defined peptides, both in preparative scale and as thousands of single peptides and peptide libraries. These can then be used in areas such as receptor research, immunology as well as for diagnostic purposes. Fully-automated, as well as semi-automated methods for the synthesis of free and resin-bound peptides, peptidomimetics and organic compounds have been developed and applied to present-day problems. New analytical methods such as pool sequencing, CZE and HPLC-MS allow the fast analysis of peptide libraries and compounds.

Publications

1. G. Jung and A. G. Beck-Sickinger (1992).
Multiple Peptide Synthesis Methods and Their Applications, Angew. Chem. 104, 375-391; Angew. Chem. Int. Ed. Engl. 31, 367-383.

2. J.W. Metzger, K.-H. Wiesmüller, V. Gnau, J. Brünjes und G. Jung (1993).
Ionspray-Massenspektrometrie und Hochleistungsflüssigkeitschromatographie - Massenspektrometrie von synthetischen Peptidbibliotheken, Angew. Chem. 105, 901-903; Angew. Chem. Int. Ed. Engl.32, 894-896.

3. S. Stevanovic and G. Jung (1993).
Multiple Sequence Analysis: Pool Sequencing of Synthetic and Natural Peptide Libraries, Anal. Biochem. 212, 212-220.

4. S. Stevanovic, K.-H. Wiesmüller, J. Metzger, A. G. Beck-Sickinger, and G. Jung (1993).
Natural and Synthetic Peptide Pools: Characterization by Sequencing and Electrospray Mass Spectrometry, Bioorg. Med. Chem. Lett. 1993, 3, 431-436.

5. J.W. Metzger, C. Kempter, K.-H. Wiesmüller, G. Jung (1994).
Electrospray Mass Spectrometry and Tandem Mass Spectrometry of Multi-Component Peptide Mixtures: Determination of Composition and Purity , Anal. Biochem. 219, 261-277.

6. J.W. Metzger, S. Stevanovic, J. Brünjes, K.-H. Wiesmüller, and G. Jung (1994).
Electrospray Mass Spectrometry and Multiple Sequence Analysis of Synthetic Peptide Libraries, Methods: A Companion of Methods in Enzymology, 6, 425-432.

7. T. Kupke, C. Kempter, G. Jung, and F. Götz (1995).
Oxidative Decarboxylation of Peptides Catalyzed by Flavoprotein Epi D: Determination of Substrate Specificity Using Peptide Libraries and Neutral Loss Mass Spectrometry, J. Biol. Chem. 270, 11282-11289.

8. G. Jung (ed.) (1996).
Combinatorial Peptide and Non-peptide Libraries (566 pages, 214 figures, 73 tables), Verlag Chemie, Weinheim.

9. K. Udaka, K.-H. Wiesmüller, S. Kienle, S. Feiertag, G. Jung, and P. Walden (1996).
Random Peptide Libraries as Tools in Basic and Applied Immunology, in: G.Jung (ed.), Combinatorial Peptide and Nonpeptide Libraries pp.349-362, Verlag Chemie, Weinheim.

10. A.G. Beck-Sickinger and G. Jung (1996).
From Multiple Peptide Synthesis to Peptide Libraries, in: G. Jung (ed.), Combinatorial Peptide and Non-peptide Libraries, pp.79-110, Verlag Chemie, Weinheim.

11. J. Früchtel and G. Jung (1996).
Organic Chemistry on Solid Supports, Angew. Chem. 108, 19-46; Angew. Chem. Int. Ed. Engl.35, 17-42.

12. K.-H. Wiesmüller, S. Feiertag, B. Fleckenstein, S. Kienle, D. Stoll, M. Herrmann, and G. Jung (1996)
Peptide and Cyclopeptide Libraries: Automated Synthesis, Analysis and Receptor Binding Assays
in: G. Jung (ed.), Combinatorial Peptide and Nonpeptide Libraries, pp. 203-246, Verlag Chemie, Weinheim.

13. J. Früchtel and G. Jung (1996)
Organic Chemistry on Solid Supports - Basic Principles for Combinatorial Chemistry - A Review in: G.Jung (ed.), Combinatorial Peptide and Nonpeptide Libraries, pp. 19-78, Verlag Chemie, Weinheim.

14. J.W. Metzger, K.-H. Wiesmüller, S. Kienle, J. Brünjes, and G. Jung (1996)
Mass Spectrometric Analysis of Peptide Libraries , in: G. Jung (ed.), Combinatorial Peptide and Nonpeptide Libraries, pp. 247-286, Verlag Chemie, Weinheim.

15. G. Jung, H. Hofstetter, S. Feiertag, D. Stoll, O. Hofstetter, K.-H. Wiesmüller, and V. Schurig (1996)
Cyclopeptide Libraries as New Chiral Selectors in Capillary Electrophoresis, Angew. Chem. 108, 2261-2263; Angew. Chem. Int. Ed. Engl. 35, 2148-2150.

16. W.J. Haap, T. Redemann, J.S. Früchtel, and G. Jung (1997)
Analytical Methods in Combinatorial Chemistry: FT-IR Spectroscopy, ATR-Spectroscopy and IR-Microscopy, GIT, Special Edition April 1997, 94-98.

cojinfo@uni-tuebingen.de(cojinfo@uni-tuebingen.de) - July 23, 1997